Colonization of Streptomyces enissocaesilis on the root system of Zea mays var ceratina
DOI:
https://doi.org/10.23917/bioeksperimen.v12i2.17484Keywords:
Metabolites, plant growth, root colonization, Streptomyces enissocaesilis, Zea mays var. ceratinaAbstract
Streptomyces enissocaesilis is a microorganism with diverse biological activities; it is capable of colonizing plant root systems by forming mycelium and adhering to root surfaces.. This study aimed to analyze the colonization of Streptomyces enissocaesilis in the root system of Zea mays var ceratina based on root infection ability, metabolite profiles in growth media, and plant vegetative growth. Zea mays var ceratina was grown hydroponically using the Deep Flow Technique (DFT) for 30 days with inoculation (1.1 × 10⁸ CFU/g) and without inoculation of Streptomyces enissocaesilis as a control. Plant growth was monitored at 7-day intervals, while infectivity and metabolite profiles were assessed at the end of the growth period; metabolite screening in the growth medium was performed using LC-MS.The results showed that Streptomyces enissocaesilis was able to infect plant root tissue, as well as interact on the root surface, as indicated by filamentous structures resembling hyphae on the epidermis of treated plant roots. Analysis of the metabolite profile in growth media inoculated with Streptomyces enissocaesilis using LC-MS revealed peaks detected at retention times of 0.72 and 1.18 minutes. Inoculation of Streptomyces enissocaesilis during the vegetative growth of Zea mays var ceratina, based on plant height and root length data, showed significantly higher values compared to the control; however, no significant differences were observed for the parameters of leaf number, dry weight, and root-to-shoot ratio. Based on these data, inoculation with Streptomyces enissocaesilis has the potential to function as a Plant Growth Promoting Rhizobacteria (PGPR). Based on the research findings, Streptomyces enissocaesilis has the potential to be developed as a plant growth promoter. However, its effectiveness in increasing biomass and overall plant growth still requires further study.
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