Integrating BSF larvae for the sustainable bioconversion of banana peels and sprout hulls waste
DOI:
https://doi.org/10.23917/bioeksperimen.v12i1.13637Keywords:
Banana peels, EM4, Organic waste bioconversion, Black Soldier Fly Larvae, Sprout hulls wasteAbstract
The management of organic waste presents a significant challenge in Indonesia, especially fruit and vegetable by-products. Black Soldier Fly (BSF, Hermetia illucens) larvae offer an effective solution due to their high potential for organic waste conversion. This study investigated the efficacy of BSF larvae in reducing and bioconverting organic waste composed of banana peels and sprout hulls, and examined the influence of EM4 fermentation on larval growth performance. Waste Reduction Index (WRI) and bioconversion rate (BCR) were calculated as percentages and analyzed descriptively, followed by the Kruskal–Wallis test. Furthermore, larval length and body weight were measured at 7, 11, 14, and 18 days of larval age and analyzed using Kruskal–Wallis with Dunn’s post-hoc test. The results showed that EM4–fermented substrates containing a 1:3 ratio of banana peels to sprout hulls showed the highest waste reduction and bioconversion potential among all mixture treatments (P < 0.05). Although larval length and weight were lower than control substrate, no significant difference in larval length was detected, while individual larval weight was significantly higher compared to other organic waste combinations (P < 0.001). In this study, EM4 fermentation enhanced lignocellulosic degradation, improving substrate digestibility for BSF larvae and optimizing bioconversion efficiency, thus supporting more sustainable organic waste management strategies.
Downloads
References
Abdirahman, R. Z., Aini, N., Ghofur, A., Wulandari, W. D., Lestari, F. K., & Putri, D. T. (2023). Studi pemanfaatan sampah organik untuk perkembangbiakan maggot di tempat pengolahan sampah terpadu (TPST) Desa Trosobo. Nusantara Community Empowerment Review, 1(1), 48-53.
Acevedo, S.A., Díaz Carrillo, Á. J., Flórez-López, E., & Grande-Tovar, C. D. (2021). Recovery of banana waste-loss from production and processing: a contribution to a circular economy. Molecules, 26(17), 5282.
Amrul, N. F., Ahmad, I. K., Basri, N. E. A., Suja, F., Jalil, N. A. A., & Azman, N. A. (2022). A review of organic waste treatment using black soldier fly (Hermetia illucens). Sustainability, 14(8), 4565.
Badan Pusat Statistik (BPS). (2023). Produksi tanaman buah-buahan, 2021-2022. Available online at https://www.bps.go.id/id/statistics-table/2/NjIjMg==/produksi-tanaman-bu ah-buahan.html, Accessed on 22 April 2024
Cahyanto, D. D., Hendrawan, Y., & Djoyowasito, G. (2019). Kinerja Pemisah Kulit Ari Tauge Kacang Hijau (Vigna radita L) Berdasarkan Amplitudo Ayakan dan Variasi Putaran. Jurnal Keteknikan Pertanian Tropis dan Biosistem, 7(3), 275-284.
Cheng, J. Y. K., Chiu, S. L. H., & Lo, I. M. C. (2017). Effects of moisture content of food waste on residue separation, larval growth and larval survival in black soldier fly bioconversion. Waste Management, 67, 315–323
Diener, S., Gutiérrez, F. R., Zurbrügg, C., & Tockner, K. L. E. M. E. N. T. (2011). Are larvae of the black soldier fly (Hermetia illucens) a financially viable option for organic waste management in Costa Rica. In Proceedings Sardinia.
Dortmans, B., Diener, S., Verstappen, B., & Zurbrügg, C. (2017). Black soldier fly bio waste processing: a step-by-step guide. Dübendorf, Switzerland: Eawag, Sandec
Firdausy, M. A., Firmansyah, M., Mizwar, A., Mahyudin, R. P., & Aurora, P. D. N. (2022). Penerapan teknologi reduksi sampah organik menggunakan black soldier fly di TPA Telang. Jurnal Pengabdian ILUNG (Inovasi Lahan Basah Unggul), 1(3), 130-136.
Food and Agriculture Organization (FAO, (2017) The Future of Food and Agriculture — Trends and Challenges. Rome: Food and Agriculture Organization of the United Nations.
Hakim, A. R., Prasetya, A., & Petrus, H. T. (2017). Studi laju umpan pada proses biokonversi limbah pengolahan tuna menggunakan larva Hermetia illucens. Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan, 12(2), 179-192.
Hamnasia, N., Muarif, A., Hakim, L., Ginting, Z., & Jalaluddin, J. (2023). Pengaruh Suhu dan Waktu Hidrolisis dalam Pembuatan Glukosa Cair Dari Ubi Jalar Kuning (Ipomoea batatas L) Menggunakan HCL. Chemical Engineering Journal Storage (CEJS), 3(6), 873-880.
Hariyono, C. M., Sriherwanto, C., Suja’i, I., Nadaviana, A., Junaedi, H., & Ma’hadah, R. (2021). Physico-chemical characteristics of Rhizopus sp.-fermented fish feed pellets containing black soldier fly larvae (Hermetia illucens) meal. In IOP Conference Series: Earth and Environmental Science (Vol. 744, No. 1, p. 012024). IOP Publishing.
Indriyanti, D. R., Widiyaningrum, P., & Setiati, N. (2023). Kombinasi pakan limbah kulit pisang dan kulit ari kedelai terhadap bobot larva Hermetia illucens (BSF) dan indeks pengurangan sampah. Life Science, 12(2), 117-127.
Isibika, A., Vinnerås, B., Kibazohi, O., Zurbrügg, C., & Lalander, C. (2021). Co-composting of banana peel and orange peel waste with fish waste to improve conversion by black soldier fly (Hermetia illucens (L.), Diptera: Stratiomyidae) larvae. Journal of Cleaner Production, 318, 128570.
Isibika, A., Vinnerås, B., Kibazohi, O., Zurbrügg, C., & Lalander, C. (2019). Pre-treatment of banana peel to improve composting by black soldier fly larvae. Waste Management, 100, 151–160.
Janusz, G., Pawlik, A., Świderska-Burek, U., Polak, J., Sulej, J., Jarosz-Wilkołazka, A., & Paszczyński, A. (2020). Laccase properties, physiological functions, and evolution. International Journal of Molecular Sciences, 21(3), 966.Kementerian PPN/BAPPENAS, 2021
Kementerian PPN/BAPPENAS. (2021). Laporan kajian food loss dan food waste di Indonesia: Dalam rangka mendukung penerapan ekonomi sirkular dan pembangunan rendah karbon. Available online at https://lcdiindonesia.id/wp-content/uploads/2021/06/Report-Kajian-FLW-F INAL4.pdf, Accessed on 15 March 2024
Kim, W. T., Bae, S. W., Park, H. C., Park, K. H., Lee, S. B., Choi, Y. C., Han, S., & Koh, Y. H. (2010). The larval age and mouth morphology of the black soldier fly, Hermetia illucens (Diptera: Stratiomyidae). International Journal of Industrial Entomology, 21(2), 185-187.
Kim, W., Bae, S., Park, K., Lee, S., Choi, Y., Han, S., & Koh, Y. (2011). Biochemical characterization of digestive enzymes in the black soldier fly, Hermetia illucens (Diptera: Stratiomyidae). Journal of Asia-Pacific Entomology, 14(1), 11-14.
Kumar, S., Negi, S., Mandpe, A., Singh, R. V., & Hussain, A. (2018). Rapid composting techniques in Indian context and utilization of black soldier fly for enhanced decomposition of biodegradable wastes-A comprehensive review. Journal of Environmental Management, 227, 189-199.
Lalander, C., Diener, S., Zurbrugg, C., & Vinneras, B. (2019). Effects of feedstock on larval development and process efficiency in waste treatment with black soldier fly (Hermetia illucens). Journal of Clean Products, 208, 211–219. https://doi.org/10.1016/j.jclepro.2018.10.017
Lee, C. M., Lee, Y. S., Seo, S. H., Yoon, S. H., Kim, S. J., Hahn, B. S., & Koo,B. S. (2014). Screening and characterization of a novel cellulase gene from the gut microflora of Hermetia illucens using metagenomic library. Journal of Microbiology and Biotechnology, 24(9), 1196-1206.
Martina, A. A., Rahayu, E. R. V., & Aprilia, L. (2025). The Utilization of Fermented Agricultural By-Products as Fibrous Feed for Beef Cattle: A Review. Bantara Journal of Animal Science, 7(2), 14-19.
Masrufah, A., Afkar, K., Fawaid, A. S., Alvarizi, D. W., Khoiriyah, L., Khoiriyah, M., & Ramadhan, M. N. (2020). Budidaya maggot BSF (Black soldier fly) sebagai pakan alternatif ikan lele (Clarias batracus) di Desa Candipari, Sidoarjo pada program holistik pembinaan dan pemberdayaan desa (PHP2D). Journal of Science and Social Development, 3(2), 10-16.
Meneguz, M., Gasco, L., & Tomberlin, J. K. (2018). Impact of pH and feeding system on black soldier fly (Hermetia illucens, L; Diptera: Stratiomyidae) larval development. PloS one, 13(8), e0202591.
Mirwandhono, R. E., Sepriadi, S., Wahyuni, T. H., & Lestari, A. (2022). An assessment of mass production and nutrient composition of Black Soldier Fly Maggot on different agriculture by-products to fermented growth media. In IOP Conference Series: Earth and Environmental Science (Vol. 1001, No. 1, p. 012011). IOP Publishing.
Mulyani, A. S., Hidayah, A. N., Paramita, D. R. A., & Azizah, S. N. (2024). Bioethanol Production by Fermentation of Rice Husk and Golden Banana Peel Delignified Using EM4. Berkala SAINSTEK, 12(1), 6-9.
Mulyati, S., Fertiasari, R., & Muslimah, M. (2020). Pemanfaatan Limbah Kulit Pisang Dalam Rangka Penguatan Ekonomi Masyarakat di Desa Sebambang Kecamatan Sambas Kabupaten Sambas. In Prosiding Seminar Akademik Tahunan Ilmu Ekonomi Dan Studi Pembangunan.
Nurfitriani, L., & Hutabarat, J. (2014). Pengaruh penambahan kotoran ayam, ampas tahu dan silase ikan rucah dalam media kultur terhadap biomassa, populasi dan kandungan nutrisi cacing sutera (Tubifex sp.). Journal of Aquaculture Management and Technology, 3(4), 109-117.
Nyakeri, E. M., Ogola, H. J. O., Ayieko, M. A., & Amimo, F. A. (2017). Valorisation of organic waste material: growth performance of wild black soldier fly larvae (Hermetia illucens) reared on different organic wastes. Journal of Insects as Food and Feed, 3(3), 193-202.
Pangestu, G. A., Pujaningsih, R. I., & Mangisah, I. (2018). Pengaruh ransum yang mengandung limbah tauge fermentasi terhadap kecernaan serat kasar, protein kasar dan energi metabolis pada itik lokal fase starter. Jurnal Ilmiah Peternakan Terpadu, 6(1), 77-82.
Permana, A. D., Susanto, A., & Giffari, F. R. (2022). Kinerja pertumbuhan larva lalat tentara hitam Hermetia illucens Linnaeus (Diptera: stratiomyidae) pada substrat kulit ari kedelai dan kulit pisang. Agrikultura, 33(1), 13-24.
Permatasari, H. R., Gulo, F., & Lesmini, B. (2014). Pengaruh konsentrasi H2SO4 dan NaOH terhadap delignifikasi serbuk bambu (Gigantochloa apus). Jurnal Penelitian Pendidikan Kimia: Kajian Hasil Penelitian Pendidikan Kimia, 1(2), 131-140.
Rachmawati, R., Buchori, D., Hidayat, P., Hem, S., & Fahmi, M. R. (2010). Perkembangan dan kandungan nutrisi larva Hermetia illucens (Linnaeus) (Diptera: Stratiomyidae) pada bungkil kelapa sawit. Jurnal Entomologi Indonesia, 7(1), 28-28.
Raksasat, R., Lim, J. W., Kiatkittipong, W., Kiatkittipong, K., Ho, Y. C., Lam, M. K., Font-Palma, C., Zaid, H. F. M & Cheng, C. K. (2020). A review of organic waste enrichment for inducing palatability of black soldier fly larvae: Wastes to valuable resources. Environmental Pollution, 267, 115488.
Rehman, K. U., Rehman, R. U., Somroo, A. A., Cai, M. M., Zheng, L. Y., Xiao, X. P., & Zhang, J. B. (2019). Enhanced bioconversion of dairy and chicken manure by the interaction of exogenous bacteria and black soldier fly larvae. Journal of Environmental Management, 237, 75–83. https://doi.org/ 10.1016/j.jenvman.2019.02.048
Rofi, D. Y., Auvaria, S. W., Nengse, S., Oktorina, S., & Yusrianti, Y. (2021). Modifikasi Pakan Larva Black Soldier Fly (Hermetia illucens) sebagai upaya percepatan reduksi sampah buah dan sayuran. Jurnal Teknologi Lingkungan, 22(1).
Romano, N., Fischer, H., Kumar, V., Francis, S. A., & Sinha, A. K. (2022). Productivity, conversion ability, and biochemical composition of black soldier fly (Hermetia illucens) larvae fed with sweet potato, spent coffee or dough. International Journal of Tropical Insect Science, 42(1), 183–190. https://doi. org/10.1007/s42690-021-00532-5
Saenab, A. (2010). Evaluasi Pemanfaatan Limbah Sayuran Pasar Sebagai Pakan Ternak Ruminansia di DKI Jakarta. Balai Pengkajian Teknologi Jakarta.Saenab, A., & Retnani, Y. 2011. Beberapa model teknologi pengolahan limbah sayuran pasar sebagai pakan alternatif pada ternak (kambing/domba) di perkotaan. In Workshop Nasional Diversifikasi Pangan Daging Ruminansia Kecil (89-96).
Saputri, M.O., Suhandoyo. (2024). The effectivityof EM4 made from bacteria Bacillus sp. for composting household waste and on the growth of Cayenne Pepper Plant (Capsicum frutescens L). Indonesian Journal of Bioscience (IJOBI) 2(1), 19-30.
Schroyen, M., Vervaeren, H., Vandepitte, H., Van Hulle, S. W., & Raes, K. (2015). Effect of enzymatic pretreatment of various lignocellulosic substrates on production of phenolic compounds and biomethane potential. Bioresource Technology, 192, 696-702.
Seberini, A. (2020). Economic, social and environmental world impacts of food waste on society and Zero waste as a global approach to their elimination. In SHS Web of Conferences (Vol. 74, p. 03010). EDP Sciences.
Siddiqui, S. A., Ristow, B., Rahayu, T., Putra, N. S., Yuwono, N. W., Mategeko, B., Smetana, S., Saki, M., Nawaz, A. & Nagdalian, A. (2022). Black soldier fly larvae (BSFL) and their affinity for organic waste processing. Waste Management, 140, 1-13.
Sihombing, S. M., & Mirwandhono, E. (2022). Maggot production in various organic wastes (vegetables, fruits, food processing industries): Potential as alternative feed substitutes for fish meal. Jurnal Peternakan Integratif, 10(2), 111-116.
Sistem Informasi Pengelolaan Sampah Nasional (SIPSN) – Kementerian Lingkungan Hidup dan Kehutanan. (2023). Capaian kinerja pengelolaan sampah dan grafik komposisi sampah. Available online at https://sipsn.menlhk.go.id/sipsn/, Accessed 28 February 2024.
Suciati, R. (2017). Efektifitas media pertumbuhan maggots Hermetia illucens (lalat tentara hitam) sebagai solusi pemanfaatan sampah organik. Biosfer: Jurnal Biologi Dan Pendidikan Biologi, 2(1), 8-13.
Suhartini, S., Sunyoto, N. M. S., Setyawan, H. Y., Rohma, N. A., Elviliana, Pratiwi, D. A., Pratiwi, Mardawati, E., Kasbawati, & Melville, L. (2024). Biomass Valorization for Bioenergy Production. In Biomass Conversion and Sustainable Biorefinery: Towards Circular Bioeconomy (67-104). Singapore: Springer Nature Singapore.
Sukowati, A., Sutikno, S., & Rizal, S. (2014). Produksi Bioetanol dari Kulit Pisang Melalui Hidrolisis Asam Sulfat [The Production of Bioetanol from Banana Peel Trough Sulphuric Acid Hidrolisis]. Jurnal Teknologi & Industri Hasil Pertanian, 19(3), 274-288.
Van Rozen, K., Elissen, H., van Wikselaar, P., Fodor, I., Federica, E., Rezaei Far, A., & Veldkamp, T. (2023). Bioconversion of different waste streams of animal and vegetal origin and manure by black soldier fly larvae Hermetia illucens L. (Diptera: Stratiomyidae). InSects, 14(2), 204.
Von Massow, M., Parizeau, K., Gallant, M., Wickson, M., Haines, J., Ma, D. W., Wallace, A., Carroll, N., & Duncan, A. M. (2019). Valuing the multiple impacts of household food waste. Frontiers in nutrition, 6, 143.
Yuktika, S., Sutiyanti, E., Dhewi, E. S., Martika, S. D., & Damas, R. (2017). Pengaruh Variasi Konsentrasi Garam terhadap Kualitas Fermentasi Udang. Bioedukasi, 10(2), 18-23.

Submitted
Accepted
Published
How to Cite
Issue
Section
License
Copyright (c) 2026 Bioeksperimen: Jurnal Penelitian Biologi

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.











