Alfalfa (Medicago sativa L.) as a Promising Herbal Galactagogue for Dairy Cows: A Review
Keywords:
Alfalfa, Bioactive compound, milk production, phytoestrogen, phyto-galactagogueAbstract
Milk production in dairy cows can be enhanced through herbal-based approaches like alfalfa (Medicago sativa L.), which is known for its richness in nutrients and bioactive compounds with the potential to act as an herbal galactagogue. This study reviews scientific publications from 2015 to 2025 in the Google Scholar database to explore the use of alfalfa and its benefits for dairy cow performance. The results indicate that alfalfa can increase milk production and quality, particularly in fat, protein, and lactose content. Phytoestrogen compounds such as isoflavones and coumestrol in alfalfa stimulate prolactin hormone production and promote udder tissue growth. Phenolic compounds help improve milk yield and quality, while alkaloids enhance the milk ejection reflex. Additionally, saponins serve as defaunation agents in the rumen, increase nitrogen utilization efficiency, and support metabolic balance, positively impacting overall cow health. However, high doses of alfalfa may lead to reproductive issues due to excessive estrogenic activity, such as anestrus and infertility. The effectiveness of alfalfa also depends on factors like material quality, dosage, the cow's physiological condition, and management practices. Therefore, alfalfa should be used carefully and in balanced amounts as a functional feed to maximize benefits and prevent physiological side effects in dairy cows.
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Abdul, N. A., & Olii, N. (2021). The Differences Effect Of Corn Hair Tea And Rajuke Tea On The Production Of Breast Milk In The Working Area Of Puskesmas Kota Selatan, Puskesma Kota Utara. Science Midwifery, 10(1), 210–216.
Abebe, R., Hatiya, H., Abera, M., Megersa, B., & Asmare, K. (2016). Bovine mastitis: Prevalence, risk factors and isolation of Staphylococcus aureus in dairy herds at Hawassa milk shed, South Ethiopia. BMC Veterinary Research, 12(1), 1–11. https://doi.org/10.1186/s12917-016-0905-3
Acharya, J. P., Lopez, Y., Gouveia, B. T., de Bem Oliveira, I., Resende, M. F. R., Muñoz, P. R., & Rios, E. F. (2020). Breeding alfalfa (Medicago sativa L.) adapted to subtropical agroecosystems. Agronomy, 10(5). https://doi.org/10.3390/agronomy10050742
Adriani. (2021). Ilmu Reproduksi Ternak Perah. Publikasi Fakultas Peternakan Universitas Jambi.
Albouchi, L., Rhouma, R. Ben, Sakhraoui, A., Ltaeif, H. B., Lanzi, F., Zribi, H., Methnani, M., Covelli, G., Hajel, O. Ben, Nsaibia, L., Ragnoni, G., & Rouz, S. (2023). A preliminary study on milk yield, composition and economic profitability of alfalfa pellets and long ryegrass fibre (Kit N°10) in a total mixed ratio in lactating dairy cows. International Journal of Veterinary Sciences and Animal Husbandry, 8(1), 01–07. https://doi.org/10.22271/veterinary.2023.v8.i1a.458
Ananda, S., Lukiwati, D. R., & Purbajanti, E. D. (2021). Production and quality of forage alfalfa (Medicago sativa L) with organic and inorganic fertilization. IOP Conference Series: Earth and Environmental Science, 788(1), 1–5. https://doi.org/10.1088/1755-1315/788/1/012072
Apostol, L., Iorga, S., Mosoiu, C., Racovita, R. C., Niculae, O. M., & Vlasceanu, G. (2017). Alfalfa Concentrate - A Rich Source of Nutrients for Use in Food Products. Agriculture & Food, 5, 66–73.
Appiah, E. A., Balla-Kovács, A., Ocwa, A., Csajbók, J., & Kutasy, E. (2024). Enhancing Alfalfa (Medicago sativa L.) Productivity: Exploring the Significance of Potassium Nutrition. Agronomy, 14(8), 1806. https://doi.org/10.3390/agronomy14081806
Bora, M., Srivastava, B., Gaidhani, S. N., Sharma, H., Gautam, M. K., Tiwari, R. K., Wanjari, M. M., Khanduri, S., & Hazra, J. (2019). Development of a Novel Polyherbal Formulation for Augmenting Milk Production in Healthy Dairy Cows. Journal of Drug Research in Ayurvedic Sciences, 4(2), 84–94. https://doi.org/10.5005/jdras-10059-0064
Burezq, H. (2022). Reproductive Ecology of Forage Alfalfa ( Medicago sativa L.): Recent Advances . Plant Reproductive Ecology - Recent Advances. https://doi.org/10.5772/intechopen.100640
Chinn, P. L. (2021). The traditional literature review. Nurse Author & Editor, 31(3–4), 62–64. https://doi.org/10.1111/nae2.29
Demir, Z., & Aksu, D. S. (2021). The relation of fat tissue hormones and some galactopoietic hormones with milk yield in Holstein and Simmental cows. Turkish Journal of Veterinary and Animal Sciences, 45(5), 901–911. https://doi.org/10.3906/VET-2012-19
El-Ramady, H., Abdalla, N., Kovacs, S., Domokos-Szabolcsy, É., Bákonyi, N., Fari, M., & Geilfus, C.-M. (2020). Alfalfa Growth under Changing Environments: An Overview. Environment, Biodiversity and Soil Security, 4(4), 201–224. https://doi.org/10.21608/jenvbs.2020.37746.1101
Elsaadawy, S. A., Wu, Z., Wang, H., Hanigan, M. D., & Bu, D. (2022). Supplementing Ruminally Protected Lysine, Methionine, or Combination Improved Milk Production in Transition Dairy Cows. Frontiers in Veterinary Science, 9(March). https://doi.org/10.3389/fvets.2022.780637
Fails, A. D., & Magee, C. (2018). Anatomy and Physiology of Farm Animals (Eighth Edi). John Willey & Sons, Inc.
Fatimah, D. N., Murod, M., Fiki, M. T., Abdillah, F., Kaswari, S., Khairunisa, I. L., Salsabila, Z. I., Juniardi, R., & Suwignyo, B. (2024). Effect of Tropical Alfalfa ( Medicago sativa L. cv Kacang Ratu BW) on the Blood Profile in Hybrid Ducks. IOP Conference Series: Earth and Environmental Science, 1360(1). https://doi.org/10.1088/1755-1315/1360/1/012009
Fernandez, A. L., Sheaffer, C. C., Tautges, N. E., Putnam, D. H., & Hunter, M. C. (2019). Alfalfa, Wildlife & the Environment (2nd ed.). National Alfalfa and Forage Alliance.
Garba, R. H., Moussa, I., Sadou, H., Suharoschi, R., & Depeint, F. (2025). Synthesis of Knowledge on Infant Fortification Plants and the Most Commonly Used Galactogenic Plants in Niger and Their Uses in the Republic of Niger. Nutraceuticals, 5, 3. https://doi.org/10.3390/nutraceuticals5010003
Gashaw, M. (2015). Review on Biomass yield Dynamics and Nutritional quality of Alfalfa (Medicago sativa). Journal of Harmionized Research in Applied Science, 3(November), 241–251. https://www.johronline.com/articles/review-on-biomass-yield-dynamics-and-nutritional-quality-of-alfalfa-medicago-sativa.pdf
Grewal, S., Sahu, J., Pal, P., Aggarwal, A., & Ghosh, S. (2019). Role of hormones in persistency of lactation: A review. Journal of Entomology and Zoology Studies, 7(2), 677–686. https://www.researchgate.net/publication/333004969
Guo, M., Wang, Z., Gao, Z., Ma, J., Huangfu, W., Niu, J., Liu, B., Li, D., Zhu, X., Sun, H., Ma, S., & Shi, Y. (2023). Alfalfa leaf meal as a new protein feedstuff improves meat quality by modulating lipid metabolism and antioxidant capacity of finishing pigs. Food Chemistry: X, 19(July), 100815. https://doi.org/10.1016/j.fochx.2023.100815
Guo, Z., Gao, S., Ouyang, J., Ma, L., & Bu, D. (2021). Impacts of heat stress-induced oxidative stress on the milk protein biosynthesis of dairy cows. Animals, 11(3), 1–14. https://doi.org/10.3390/ani11030726
Hayati, L. N., Tyasningsih, W., Praja, R. N., Chusniati, S., Yunita, M. N., & Wibawati, P. A. (2019). Isolasi dan Identifikasi Staphylococcus aureus p. Jurnal Medik Veteriner, 2(2), 76.
Javaid, T., Sadaqat, H. A., Iqbal, M. A., & Wahid, M. A. (2020). Development of high forage yield and better-quality alfalfa population. Pakistan Journal of Agricultural Sciences, 57(3), 691–699. https://doi.org/10.21162/PAKJAS/20.10018
Kalaimathi, & Kingsly, A. (2019). Review on Plants with Galactogouge activity. International Journal of Reverse Pharmacology and Health Research (IJRPHR), 2(3), 65–67. https://doi.org/10.121/ijrphr/02.0204.374
Karayilanli, E., & Ayhan, V. (2016). Investigation of feed value of alfalfa (Medicago sativa L.) harvested at different maturity stages. Legume Research, 39(2), 237–247. https://doi.org/10.18805/lr.v0iOF.9292
Khan, N. (2020). Critical Review of Dairy Cow Industry in The World. University of Agriculture, Peshawar. https://dx.doi.org/10.2139/ssrn.3564129
Khan, S., Manjusha, K. M., S, A. B., Haritha, C. V, Kumar, R., Pawde, A. M., & Amarpa. (2020). Surgical Affections of Udder and Teats in Large Ruminants. Raksha Techincal Review, 9(2), 16–21.
Kholif, A. E. (2023). A Review of Effect of Saponins on Ruminal Fermentation, Health and Performance of Ruminants. Veterinary Sciences, 10, 450. https://doi.org/10.3390/vetsci10070450
Koko, B. K., Konan, A. B., Kouacou, F. K. A., Djétouan, J. M. K., & Amonkan, A. K. (2019). Galactagogue Effect of Euphorbia hirta (Euphorbiaceae) Aqueous Leaf Extract on Milk Production in Female Wistar Rats. Journal of Biosciences and Medicines, 07(09), 51–65. https://doi.org/10.4236/jbm.2019.79006
Kulkarni, M. B., & Jamdade, R. S. (2017). Conceptual study of stanapida W . S . R . ( with special reference ) to breast congestion in Galactopoiesis. International Journal of Multidisciplinary Research and Development, 4(4), 30–33.
Kumai, A., Tsugami, Y., Wakasa, H., Suzuki, N., Suzuki, T., Nishimura, T., & Kobayashi, K. (2020). Adverse Effects of Coumestrol and Genistein on Mammary Morphogenesis and Future Milk Production Ability of Mammary Epithelial Cells. Advanced Biosystems, 4(4), 1–13. https://doi.org/10.1002/adbi.201900187
Kumar, S., Kumar, A., Nayal, S., Shukla, A., & Kailkhura, S. (2023). A Comprehensive Review on Possible Synergistic Therapeutic Effects and Comparison Between Phytochemical and Nutritional Profile of Medicago sativa and Panax ginseng . Pharmacognosy Magazine, 19(4), 799–810. https://doi.org/10.1177/09731296231197306
Latif, A., Sun, Y., & Noman, A. (2023). Herbaceous Alfalfa plant as a multipurpose crop and predominant forage specie in Pakistan. Sustainable Food Systems, 7(1126151), 1–12. https://doi.org/10.3389/fsufs.2023.1126151
Li, S., & Wang, H. (2018). Traditional Literature Review and research Synthesis. In The Palgrave Handbook of Applied Linguistics Research Methodology (pp. 123–144). https://doi.org/10.1057/978-1-137-59900-1
Liu, L., Liang, T., Liang, J., Yang, L., Xu, Z., Liu, G., & Li, J. (2023). Evaluation of different Alfalfa cultivar ’ s hay yield and nutritional evaluation at amino acids level. Pakistan Journal of Agricultural Sciences, 60(2), 407–414. https://doi.org/10.21162/PAKJAS/23.247
Ma, J., Fan, X., Sun, G., Yin, F., Zhou, G., Zhao, Z., & Gan, S. (2024). Replacing alfalfa hay with amaranth hay: effects on production performance, rumen fermentation, nutrient digestibility and antioxidant ability in dairy cow. Animal Bioscience, 37(2), 218–227. https://doi.org/10.5713/ab.23.0232
Ma, J., Huangfu, W., Yang, X., Xu, J., Zhang, Y., Wang, Z., Zhu, X., Wang, C., Shi, Y., & Cui, Y. (2022). “King of the forage”—Alfalfa supplementation improves growth, reproductive performance, health condition and meat quality of pigs. Frontiers in Veterinary Science, 9(1025942), 1–13. https://doi.org/10.3389/fvets.2022.1025942
Mandle, G., & Chaudhari, V. (2021). Ashwabala (Medicago sativa Linn.) Nari Aushadhi - A Review. Journal of Ayurveda and Integrated Medical Sciences, 5(5), 315–325.
Marques, R. O., Gonçalves, H. C., de Lima Meirelles, P. R., de Paula Ferreira, R., Gomes, H. F. B., Lourençon, R. V., Brito, E. P., & Cañizares, G. I. L. (2022). Production, intake, and feeding behavior of dairy goats fed alfalfa via grazing and cassava. Revista Brasileira de Zootecnia, 51(e20210102), 1–11. https://doi.org/10.37496/rbz5120210102
Mohammed, Z. A., & Sanahmmed, A. M. (2024). “Effect of Phytoestrogens on Some Reproductive Physiology and Performance.” Journal of Veterinary Medicine and Animal Sciences, 7(1), 1145.
Monirifar, H., Mirmozaffari, R. A., Ghassemi, S., & Tavasolee, A. (2020). Harvest Time and Cultivar Effects on Growth, Physiological Traits, Yield and Quality of Alfalfa in Saline Condition. International Journal of Plant Production, 14(3), 453–462. https://doi.org/10.1007/s42106-020-00096-3
Muhammad, G., Rashid, I., Firyal, S., & Saqib, M. (2016). Lactation in Dry Barren ( Infertile ) Dairy Cows and Buffaloes : A Mini Review. Scholar’s Advances in Animal and Veterinary Research, 2(4), 205–211.
Nanditha, R. J., & Varun, H. S. (2022). Herbal Galactogogues – A Booster for Milk Production in Dairy Animals. Justagriculture.In, 3(3), 1–5. https://justagriculture.in/files/newsletter/2022/november/16. Herbal Galactogogues – A Booster for Milk Production in Dairy Animals.pdf
Nasihin, A., & Fadhlurrohman, I. (2025). Potential of Functional Food Fortified Alfalfa (Medicago sativa L.) in Fermented Milk Products: A Literature Review. Jurnal Keteknikan Pertanian Tropis Dan Biosistem, 13(1), 52–63. https://doi.org/10.21776/ub.jkptb.2025.013.01.05
Neela, R., Prabakaran, R., & Neelavathy, R. (2016). a Review of Galactagogues in Siddha System of Medicine. International Journal of Research in Pharmaceutical and Nano Sciences, 5(3), 140–144.
Pahuja, A., Jain, M., & Rawat, K. (2024). A Review on Galactogogic Properties of India’s Rich Tradition of Medicinal Herbs and Spices for Lactation. Traditional and Integrative Medicine, 9(3), 308–317. https://doi.org/10.18502/tim.v9i3.16535
Patra, P. S., & Paul, T. (2019). Lucerne (Alfalfa). In M. Hedayetullah & P. Zaman (Eds.), Forage Crops of the World (1st Editio, p. 231). Apple Academic Press, Inch.
Posan, P., Suler, A., Nistor, L., Hodosan, C., Marius, M., & Udroiu, A. (2023). Use of Medicinal Plants with Galactogenic Effect, as a Food Supplement, in Order to Increase Milk Production, in Dairy Animals: A Review. Indian Journal of Animal Research, 57(6), 677–683. https://doi.org/10.18805/IJAR.BF-1527
Pratiwi, Y. S., Handayani, S., & Fatmawati, N. (2023). Identification Of Alkaloids And Steroids In Moringa Oleifera Leaves As A Breastfeeding. Jurnal Kebidanan Malahayati, 9(1), 53–57. https://doi.org/10.33024/jkm.v9i1.8510
Qingbin, W., & Yang, Z. (2020). China’s alfalfa market and imports: Development, trends, and potential impacts of the U.S.–China trade dispute and retaliations. Journal of Integrative Agriculture, 19(4), 1149–1158. https://doi.org/10.1016/S2095-3119(19)62832-7
Raju, K., K, M., Vankayala, J., Kammardi, S., Veeranna, V., C, K., Rajeshwari, R., B, Y., Nagaraj, N., S, C., & J, S. (2018). Intensive Cultivation of Medicago sativa for Sustainable Milk Production - An Action Oriented Approach. International Journal of Livestock Research, 8(4), 1. https://doi.org/10.5455/ijlr.20171017055204
Rani, M., Pathak, A., Goel, M., Hossain, E., Shivakumar, P., Kumar, S., & Pathak, V. (2024). Herbal galactagogues : exploring plants enhancing lactation in postpartum women. African Journal of Biomedical Research, 27(4), 1732–1737.
Rezaei, R., Wu, Z., Hou, Y., Bazer, F. W., & Wu, G. (2016). Amino acids and mammary gland development: Nutritional implications for milk production and neonatal growth. Journal of Animal Science and Biotechnology, 7(1). https://doi.org/10.1186/s40104-016-0078-8
Roy, A. K., Malaviya, D. R., & Kaushal, P. (2016). Genetic improvement of fodder legumes especially dual purpose pulses. Indian Journal of Genetics and Plant Breeding, 76(4), 608–625. https://doi.org/10.5958/0975-6906.2016.00076.6
Safitri, L., Syamsi, A. N., Setiana, L., & Nuskhi, M. (2020). Susu Ternak Dalam Bingkai Tafsir ‘Ilmi: Studi Integrasi Tafsir Al-Quran Dan Ilmu Peternakan. Prosiding Seminar Teknologi Agribisnis Peternakan (Stap) Fakultas Peternakan Universitas Jenderal Soedirman, 7, 184–201. http://jnp.fapet.unsoed.ac.id/index.php/psv/article/view/477
Sahu, U., Shah, K., & Chauhan, N. S. (2022). Potential Galactogogues: A Review. International Journal of Pharmaceutical Sciences and Nanotechnology, 15(1), 5726–5740. https://doi.org/10.37285/ijpsn.2022.15.1.2
Salum, Ç., & Etyemez, M. (2023). A Physiological Perspective on Lactation in Goats: A Review. Bozok Veterinary Sciences, 4(2), 65–72. https://doi.org/10.58833/bozokvetsci.1393630
Sanghai, A. A., Bhojne, G. R., Dakshinkar, N. P., Dhoot, V. M., & DUbey, A. G. (2023). Evaluation of a poly: Herbal Galactagogue on milk production in buffaloes. International Journal of Veterinary Sciences and Animal Husbandry, 8(6), 139–141.
Scholz-Ahrens, K. E., Ahrens, F., & Barth, C. A. (2020). Nutritional and health attributes of milk and milk imitations. European Journal of Nutrition, 59(1), 19–34. https://doi.org/10.1007/s00394-019-01936-3
Shah, M. S., Supriya, D., Mayuri, G., & Oswal, R. J. (2020). A Systematic review on one of the nutraceutical potential plant medicago sativa (alfalfa). International Journal of Research and Analytical Reviews, 7(1), 608–616. https://doi.org/10.20959/wjpr20209-18453
Sharifi, M., Hosseinkhani, A., Sofizade, M., & Mosavi, J. (2016). Effects of fat supplementation and chop length on milk composition and ruminal fermentation of cows fed diets containing alfalfa silage. Iranian Journal of Applied Animal Science, 6(2), 293–301.
Shkirin, A. V., Ignatenko, D. N., Chirikov, S. N., Bunkin, N. F., Astashev, M. E., & Gudkov, S. V. (2021). Analysis of fat and protein content in milk using laser polarimetric scatterometry. Agriculture (Switzerland), 11, 1028. https://doi.org/10.3390/agriculture11111028
Singh, A. K., Bhakat, C., Kisku, U., Karunakaran, M., & Dutta, T. K. (2025). Effect of supplementation of phytoadditive mixtures on live weight, blood indices, hormonal profile and reproductive performance in Black Bengal goats. Scientific Reports, 15(1), 1–12. https://doi.org/10.1038/s41598-025-89026-9
Sogunle, T., Medical, F., Abeokuta, C., & Sogunle, E. (2023). Traditional Literature Review Versus Systematic Literature Review in the Context of Evidence -Based Medicine. Nigeria Journal of Family Practice, 11 No. 5(April), 9–12. https://www.researchgate.net/publication/370204728%0ATRADITIONAL
Srisaikham, S., & Rupitak, Q. (2021). A Preliminary Study on Growth, Yield and Nutritive Value of Four Varieties of Alfalfa and the Utilization of Alfalfa Dehydrated Pellets in a Total Mixed Ratio in Meat Goat Diet. Chiang Mai University Journal of Natural Sciences, 20(1), 1–17. https://doi.org/10.12982/CMUJNS.2021.003
Suwignyo, B., Aristia Rini, E., & Helmiyati, S. (2023). The profile of tropical alfalfa in Indonesia: A review. Saudi Journal of Biological Sciences, 30(1), 103504. https://doi.org/10.1016/j.sjbs.2022.103504
Thakur, M., Khedkar, R., Singh, K., & Sharma, V. (2023). Ethnopharmacology of Botanical Galactagogues and Comprehensive Analysis of Gaps Between Traditional and Scientific Evidence. Current Research in Nutrition and Food Science, 11(2), 589–604. https://doi.org/10.12944/CRNFSJ.11.2.11
Tomovska, J., Vllasaku, I., & Josevska, E. (2023). Chemical Composition of Animal Feed and Its Influence on the Milk Quality. Food and Environment Safety Journal, 22(2), 122–134. https://doi.org/10.4316/fens.2023.012
Truchet, S., & Honvo-Houéto, E. (2017). Physiology of milk secretion. Best Practice & Research Clinical Endocrinology & Metabolism, 31(4), 367–384. https://doi.org/10.1016/j.beem.2017.10.008.
Wang, T., & Zhang, W. H. (2023). Priorities for the development of alfalfa pasture in northern China. Fundamental Research, 3(2), 225–228. https://doi.org/10.1016/j.fmre.2022.04.017
Winnicki, S., Jugowar, J., Aerts, J., & Sobek, Z. (2017). The effect of milking systems on the quantity and quality of cow milk. Journal of Research and Applications in Agricultural, 62(4), 193–196.
Wyse, J., Latif, S., Gurusinghe, S., McCormick, J., Weston, L. A., & Stephen, C. P. (2022). Phytoestrogens: A Review of Their Impacts on Reproductive Physiology and Other Effects upon Grazing Livestock. Animals, 12, 2709. https://doi.org/10.3390/ani12192709
Wyse, J. M., Latif, S., Gurusinghe, S., Berntsen, E. D., Weston, L. A., & Stephen, C. P. (2021). Characterization of phytoestrogens in medicago sativa l. And grazing beef cattle. Metabolites, 11(8). https://doi.org/10.3390/metabo11080550
Xu, W., Zhang, Y., & Zhang, L. (2018). Research progress on the function and analytical determination technology of active constituents in alfalfa. Journal of Northern Agriculture, 46(4), 128–134. https://doi.org/10.3969/j.issn.2096-1197.2018.04.23
Zhan, J., Liu, M., Su, X., Zhan, K., Zhang, C., & Zhao, G. (2017). Effects of alfalfa flavonoids on the production performance, immune system, and ruminal fermentation of dairy cows. Asian-Australasian Journal of Animal Sciences, 30(10), 1416–1424. https://doi.org/10.5713/ajas.16.0579
Zigo, F., Vasil’, M., Ondrašovičová, S., Výrostková, J., Bujok, J., & Pecka-Kielb, E. (2021). Maintaining Optimal Mammary Gland Health and Prevention of Mastitis. Frontiers in Veterinary Science, 8(February), 1–17. https://doi.org/10.3389/fvets.2021.607311.
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