Optimization of Microgel Formula Combination of Snail Mucus and Catechin to Accelerate Wound Closure
DOI:
https://doi.org/10.23917/pharmacon.v21i2.7562Keywords:
Design Expert, Microgel, Optimization, Snail Mucus-Catechin, Wound closureAbstract
Incised wound healing is a crucial process that requires effective treatment solutions. A microgel containing snail mucus and catechin seems a promising approach to accelerate healing and improve recovery outcomes. This study aimed to develop a microgel preparation contain snail mucus (Achatina fulica) and catechin. Formulation design is assisted using the Design Expert SLD method. The optimum formula was achieved with a carbopol to TEA ratio of 1.589:0.411, with estimated values of pH (4.506), viscosity (18,873 cPs), spread ability (5.477 cm), and adhesion (2.068 second). These estimated values showed no significant difference from the actual values obtained in the laboratory experiments. To evaluate the quality of the preparation, in vivo testing was performed on white mice, and the data were analysed using one-way ANOVA. The snail mucus and catechin microgels were proven to be effective in treating deep incision wounds.
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Baby, J. N., Pramod, K., John, S., Aji Alex, M. R., Suneesh, C. V., Kapoor, S., & Tahir, M. A. (2015). Preparation and Evaluation of Tunable Emission Zinc Oxide Quantum Dots for Gene Delivery. International Journal of Pharmaceutical Sciences Review and Research, 35(1), 191–194. https://globalresearchonline.net/journalcontents/v35-1/36.pdf
Dooley, A., Bruckdorfer, K. R., & Abraham, D. J. (2012). Modulation of Fibrosis in Systemic Sclerosis by Nitric Oxide and Antioxidants. Cardiology Research and Practice, 1(1), 191-194. https://doi.org/10.1155/2012/521958 DOI: https://doi.org/10.1155/2012/521958
Gaikwad, S., Birla, S., Ingle, A. P., Gade, A., Ingle, P., Golińska, P., & Rai, M. (2022). Superior in Vivo Wound-Healing Activity of Mycosynthesized Silver Nanogel on Different Wound Models in Rat. Frontiers in Microbiology, 13, 1–16. https://doi.org/10.3389/fmicb.2022.881404 DOI: https://doi.org/10.3389/fmicb.2022.881404
Handayani, F. S., Nugroho, B. H., & Munawiroh, S. Z. (2018). Optimization of Low Energy Nanoemulsion of Grape Seed Oil Formulation Using D-Optimal Mixture Design (DMD). Jurnal Ilmiah Farmasi, 14(1), 17–34. http://journal.uii.ac.id/index.php/JIF DOI: https://doi.org/10.20885/jif.vol14.iss1.art03
Komalasari, K. W., Ketut Kwartantaya Winaya, Putu Gde Hari Wangsa, & I Dewa Made Rendy Sanjaya. (2022). Keloids Treated with a Combination of Surgical Excision and Intralesional Corticosteroid Injection: a Case Report. Intisari Sains Medis, 13(3), 561–565. https://doi.org/10.15562/ism.v13i3.1504 DOI: https://doi.org/10.15562/ism.v13i3.1504
Marsili, L., Dal Bo, M., Berti, F., & Toffoli, G. (2021). Thermoresponsive Chitosan-Grafted-Poly(N-Vinyl Caprolactam) Microgels Via Ionotropic Gelation For Oncological Applications. Pharmaceutics, 13(10), 1–26. https://doi.org/10.3390/pharmaceutics13101654 DOI: https://doi.org/10.3390/pharmaceutics13101654
Murdiana, H. E., Putri, M. K., Rosita, M. E., Kristariyanto, Y. A., & Kurniawaty, A. Y. (2022). Optimization of Rice (Oryza Sativa L.) Type M/a Cream Dosage Formula with Variations of Stearic Acid, Cetyl Alcohol and Triethanolamine. Jurnal Farmamedika (Pharmamedica Journal), 7(2), 55–63. https://doi.org/10.47219/ath.v7i2.161 DOI: https://doi.org/10.47219/ath.v7i2.161
Nilawati Usman, A., Sartini, S., Yulianti, R., Kamsurya, M., Oktaviana, A., Nulandari, Z., Agustin, D. I., & Fendi, F. (2024). Turmeric extract gel and honey in post-cesarean section wound healing: A preliminary study. F1000Research, 12, 1–14. https://doi.org/10.12688/f1000research.134011.2 DOI: https://doi.org/10.12688/f1000research.134011.2
Nugroho, A. A., Adianto, C., & Patria, Y. (2020). Nano-Androcerum: Wound Healing Gel Innovation from Binahong Leaf and Cinnamon Nanoparticles on Chronic Wounds. Bimfi, 7(1), 26–42. https://doi.org/https://doi.org/10.48177/bimfi.v7i1.11 DOI: https://doi.org/10.48177/bimfi.v7i1.11
Ousey, K., Djohan, R., Dowsett, C., Ferreira, F., Hurd, T., & Romanelli, M. (2018). Surgical Wound Dehiscence: Improving Prevention and Outcomes. World Union of Wound Healing Societies. Consensus Document, 4. 17-19. https://pure.hud.ac.uk/en/publications/surgical-wound-dehiscence-improving-prevention-and-outcomes
Pradita, E. Y., & Wahyuni, S. (2023). Nanogel Synthesis Of Chitosan-Alginate-Siam Orange (Citrus nobilisLour) Extract and Its Antibacterial Activity. Indonesian Journal of Chemical Science, 12(1), 58–69. https://doi.org/10.15294/ijcs.v12i1
Pudiastuti, & Aisiyah, S. (2014). Gel Formulation Combination Snail Slime (Achatina fulica Ferr) and Aloe vera as Active Material for Treatment of Burn. Jurnal Farmasi Indonesia, 11(2), 123–129. https://doi.org/10.31001/jfi.v11i2.63
Rahmatullah, S. . A. W. . & K. N. (2020). Effect of Various Concentrations of Hydroxy Propyl Methyl Cellulose (HPMC) on the Physical Stability of Tobacco Extract Gel (Nicotiana tabaccum L.) and its Activity against Streptococcus mutans. CHMK Pharmaceutical Scientific Journal, 3(3), 189–194. https://cyber-chmk.net/ojs/index.php/farmasi/article/view/847
Rohmani, S., & Kuncoro, M. A. A. (2019). Stability and Activity Test of Basil Leaf Extract Gel and Sanitizer. JPSCR : Journal of Pharmaceutical Science and Clinical Research, 4(1), 16-28. https://doi.org/10.20961/jpscr.v4i1.27212 DOI: https://doi.org/10.20961/jpscr.v4i1.27212
Rowe, R. C. R., Sheskey, P. J. S., & Cook, W. (2009). Handbook Pharmaceutical Excipients, Sixth Edition. 1064. https://adiyugatama.wordpress.com/wp-content/uploads/2012/03/handbook-of-pharmaceutical-excipients-6th-ed.pdf
Shoviantari, F., Fajriyah, S., Agustin, E., & Khairani, S. (2021). Activity Test of Snail Slime Gel (Achatina Fulica) as Wound Healing. Jurnal Ilmiah As-Syifaa, 13(1), 12–19. https://doi.org/10.33096/jifa.v13i1.648 DOI: https://doi.org/10.33096/jifa.v13i1.648
Song, Y., Wang, T., Yang, L., Wu, J., Chen, L., Fan, X., Zhang, Z., Yang, Q., Yu, Z., & Song, B. (2023). EGCG Inhibits Hypertrophic Scar Formation in a Rabbit Ear Model. Journal of Cosmetic Dermatology, 22(4), 1382–1391. https://doi.org/10.1111/jocd.15587 DOI: https://doi.org/10.1111/jocd.15587
Srivastava, S., Saha, S., & Jakhmola, V. (2023). Nanogel: Types, Methods of Preparation, Limitation, Evaluation and Application-A Systematic Review. International Journal of Drug Delivery Technology, 13(4), 1631–1639. https://doi.org/10.25258/ijddt.13.4.77 DOI: https://doi.org/10.25258/ijddt.13.4.77
Tsabitah, A. F., Zulkarnain, A. K., Wahyuningsih, M. S. H., & Nugrahaningsih, D. A. A. (2020). Optimization of Carbomer, Propylene Glycol, and Triethanolamine in the Formulation of Gel Preparation of Ethanol Extract of Moon Flower Leaf (Tithonia diversifolia). Majalah Farmaseutik, 16(2), 111-118. https://doi.org/10.22146/farmaseutik.v16i2.45666 DOI: https://doi.org/10.22146/farmaseutik.v16i2.45666
Yati, K., Jufri, M., Gozan, M., & Dwita, L. P. (2018). Effect of Various Concentrations of Hydroxy Propyl Methyl Cellulose (HPMC) on the Physical Stability of Tobacco Extract Gel (Nicotiana tabaccum L.) and its Activity against Streptococcus mutans. Pharmaceutical Sciences and Research, 5(3), 133–141. https://doi.org/10.7454/psr.v5i3.4146 DOI: https://doi.org/10.7454/psr.v5i3.4146
