Irritation, Hedonic, and Antioxidant Activity Testing of Sunkist Orange Peel (Citrus sinensis (L.) Osbeck) Peel-Off Gel Mask Formulation
DOI:
https://doi.org/10.23917/pharmacon.v21i2.3130Keywords:
Sunkist orange peel (Citrus sinensis (L.) Osbeck), Peel-off gel mask, Antioxidant activity, sensory evaluation, primary irritation index (PII)Abstract
Sunkist orange peel is a rich source of antioxidants beneficial for skin protection, making it a promising ingredient in cosmetic products like peel-off gel masks. This study investigated the sensory profile, primary irritation index (PII), and antioxidant activity of peel-off gel mask formulations containing Sunkist orange peel extract (Citrus sinensis (L.) Osbeck). The extract was obtained through maceration using 96% ethanol, with a mean yield of 9.95 ± 1,2 %. Phytochemical analysis identified the presence of polyphenols, tannins, and flavonoids. The extract was incorporated into peel-off gel mask formulations and evaluated for quality parameters (organoleptic properties, pH, viscosity, and drying speed), sensory characteristics, PII, and antioxidant activity using the DPPH method. All formulations fulfilled the required quality standards. Sensory evaluation indicated that Formula III (FIII) was the most favored by respondents across all stages of application (before, during, and after use). The irritation index test on 25 volunteers revealed no irritation, with PII values ranging from 0 to 0.4, classifying the preparations as non-irritating. Antioxidant evaluation showed that the peel-off gel with 2% extract exhibited an IC-50 value of 23.38 ppm, categorizing it as a very strong antioxidant. These findings highlight the potential of Sunkist orange peel extract in peel-off gel masks, combining excellent antioxidant properties with favorable sensory and safety profiles. Further optimization may enhance its application in skincare products.
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Ali, M. A. M., & Kaid, B. M. (2024). Development and characterization of peel-off gel masks incorporating activated charcoal and salicylic acid for enhanced skin radiance and rejuvenation. International Journal of Pharmaceutical Sciences, 2 (8), 3024-3034. https://doi.org/10.5281/zenodo.13294605
Andrade, D. M., & Fasolo, D. (2014). Polyphenol antioxidants from natural sources and contribution to health promotion. In R. R. Watson, V. R. Preedy, & S. Zibadi (Eds.), Polyphenols in human health and disease. Academic Press, 253-265. https://doi.org/10.1016/B978-0-12-398456-2.00020-7 DOI: https://doi.org/10.1016/B978-0-12-398456-2.00020-7
Anjani, D. (2018). Formulation and antioxidant activity test of peel-off mask preparation of lemon peel essential oil (Citrus limon L.) [Bachelor's thesis]. UIN Syarif Hidayatullah Jakarta.
Athaillah, A., Sitorus, A., Rambe, R., Pangondean, A., & Chandra, P. (2022). Formulation and evaluation of sheet mask containing green apple fruit (Malus domestica) extract as antioxidant. Journal of Pharmaceutical And Sciences, 5(1), 54-61. https://doi.org/10.36490/journal-jps.com.v5i1.100 DOI: https://doi.org/10.36490/journal-jps.com.v5i1.100
Binder, L., Mazál, J., Petz, R., Klang, V., & Valenta, C. (2019). The role of viscosity on skin penetration from cellulose ether-based hydrogels. Skin Research and Technology, 25(5), 725-734. https://doi.org/10.1111/srt.12709 DOI: https://doi.org/10.1111/srt.12709
Burke, K. E. (2018). Mechanisms of aging and development: A new understanding of environmental damage to the skin and prevention with topical antioxidants. Mechanisms of Ageing and Development, 172, 123-130. https://doi.org/10.1016/j.mad.2017.12.003 DOI: https://doi.org/10.1016/j.mad.2017.12.003
Cahnia, M. S., Muhaimin, M., & Yuliawati, Y. (2022). Formulation, effectiveness test and hedonic test of peel off gel mask combination of turmeric rhizome extract (Curcuma longa L.) and honey (Mel depuratum) as a skin elasticity enhancer. Jurnal Ilmiah Kefarmasian Medical Sains, 7(1), 23-36. https://doi.org/10.37874/ms.v7i2.328 DOI: https://doi.org/10.37874/ms.v7i2.328
Chang, S. K., Alasalvar, C., & Shahidi, F. (2016). Review of dried fruits: Phytochemicals, antioxidant efficacies, and health benefits. Journal of Functional Foods, 21, 113-132. https://doi.org/10.1016/j.jff.2015.11.034 DOI: https://doi.org/10.1016/j.jff.2015.11.034
Dănilă, E., Kaya, M., Violeta, M., & Öztürk, Ş. (2020). Formulation and characterization of anti-aging cosmetic emulsions based on collagen hydrolysate and caffeine. In Proceedings of the 8th International Conference on Advanced Materials and Systems, 139-144. https://doi.org/10.24264/icams-2020.II.6 DOI: https://doi.org/10.24264/icams-2020.II.6
Depari, S. A. F., Rambe, D. J. A., & Meilando, R. (2021). Effectiveness test of ethanol extract of Sunkist orange peel (Citrus sinensis (L.) Osbeck) on blood sugar levels of Wistar rats (Rattus norvegicus) with streptozotocin-induced hypercholesterolemia. Biospecies, 14(1), 1-9. https://doi.org/10.22437/biospecies.v14i1.11250 DOI: https://doi.org/10.22437/biospecies.v14i1.11250
Dewi, A. L., Siregar, V. D., & Kusumayanti, H. (2019). Effect of extraction time on tannin antioxidant level and flavonoid on pandan wangi leaf (Pandanus amaryllifolius Roxb) using hydrothermal extractor. Journal of Physics: Conference Series, 1295(1), Article 012066. https://doi.org/10.1088/1742-6596/1295/1/012066 DOI: https://doi.org/10.1088/1742-6596/1295/1/012066
Guzmán, E., & Lucia, A. (2021). Essential oils and their individual components in cosmetic products. Cosmetics, 8(4), Article 114. https://doi.org/10.3390/cosmetics8040114 DOI: https://doi.org/10.3390/cosmetics8040114
Haitami, H., Ulfa, A., & Muntaha, A. (2017). Vitamin C content of Sunkist orange juice and infused water. Medical Laboratory Technology Journal, 3(1), 22-26. https://doi.org/10.31964/mltj.v3i1.149 DOI: https://doi.org/10.31964/mltj.v3i1.149
Karnelo, R., Pudiarifanti, N., & Jatiningsih, S. (2021). Formulation of gel peel off facial mask preparation from lime peel ethanol extract (Citrus aurantiifolia). Journal of Pharmaceutical Care, 1(1), 45-52. https://doi.org/10.33088/jp.v1i1.138 DOI: https://doi.org/10.33088/jp.v1i1.138
Kose, O., Erkekoglu, P., Sabuncuoglu, S., & Kocer-Gumusel, B. (2018). Evaluation of skin irritation potentials of different cosmetic products in Turkish market by reconstructed human epidermis model. Regulatory Toxicology and Pharmacology, 98, 268-273. https://doi.org/10.1016/j.yrtph.2018.08.010 DOI: https://doi.org/10.1016/j.yrtph.2018.08.010
Kurihara-Bergstrom, T., Knutson, K., Denoble, L. J., & Goates, C. Y. (1990). Percutaneous absorption enhancement of an ionic molecule by ethanol-water systems in human skin. Pharmaceutical Research, 7(7), 762-766. https://doi.org/10.1023/A:1015879925147 DOI: https://doi.org/10.1023/A:1015879925147
Long, X., Zeng, X., Yan, H., Xu, M., Zeng, Q., Xu, C., Xu, Q., Liang, Y., & Zhang, J. (2021). Flavonoids composition and antioxidant potential assessment of extracts from Gannanzao navel orange (Citrus sinensis Osbeck cv. Gannanzao) peel. Natural Product Research, 35(4), 702-706. https://doi.org/10.1080/14786419.2019.1593162 DOI: https://doi.org/10.1080/14786419.2019.1593162
Panche, A. N., Diwan, A. D., & Chandra, S. R. (2016). Flavonoids: An overview. Journal of Nutritional Science, 5, Article e47. https://doi.org/10.1017/jns.2016.41 DOI: https://doi.org/10.1017/jns.2016.41
Prommaban, A., & Chaiyana, W. (2022). Microemulsion of essential oils from Citrus peels and leaves with anti-aging, whitening, and irritation reducing capacity. Journal of Drug Delivery Science and Technology, 69, Article 103188. https://doi.org/10.1016/j.jddst.2022.103188 DOI: https://doi.org/10.1016/j.jddst.2022.103188
Rekha, C., Poornima, G., Manasa, M., Abhipsa, V., Devi, J. P., Kumar, H. T. V., & Kekuda, T. R. P. (2012). Ascorbic acid, total phenol content and antioxidant activity of fresh juices of four ripe and unripe citrus fruits. Chemical Science Transactions, 1(2), 303-310. https://doi.org/10.7598/cst2012.182 DOI: https://doi.org/10.7598/cst2012.182
Saini, A., Panesar, P. S., & Bera, M. B. (2019). Comparative study on the extraction and quantification of polyphenols from Citrus peels using maceration and ultrasonic technique. Current Research in Nutrition and Food Science, 7(3), 678-685. https://doi.org/10.12944/crnfsj.7.3.08 DOI: https://doi.org/10.12944/CRNFSJ.7.3.08
Santoso, I., Prayoga, T., Agustina, I., & Rahayu, W. S. (2020). Formulation of Aloe vera (Aloe vera L.) peel-off gel mask with polyvinyl alcohol gelling agent. Jurnal Riset Kefarmasian Indonesia, 2(1), 17-25. https://doi.org/10.33759/jrki.v2i1.33 DOI: https://doi.org/10.33759/jrki.v2i1.33
Schulte to Brinke, A., Mehlich, A., Doberenz, C., & Janssens-Böcker, C. (2021). Acidification of the skin and maintenance of the physiological skin pH value by buffered skin care products formulated around pH 4. Journal of Cosmetics, Dermatological Sciences and Applications, 11(1), 44-57. https://doi.org/10.4236/jcdsa.2021.111005 DOI: https://doi.org/10.4236/jcdsa.2021.111005
Simmonds, G., Woods, A. T., & Spence, C. (2018). 'Show me the goods': Assessing the effectiveness of transparent packaging vs. product imagery on product evaluation. Food Quality and Preference, 63, 18-27. https://doi.org/10.1016/j.foodqual.2017.07.015 DOI: https://doi.org/10.1016/j.foodqual.2017.07.015
Suseela, V. (2019). Potential roles of plant biochemistry in mediating ecosystem responses to warming and drought. In J. E. Mohan (Ed.), Ecosystem consequences of soil warming. Academic Press, 103-124. https://doi.org/10.1016/B978-0-12-813493-1.00005-3 DOI: https://doi.org/10.1016/B978-0-12-813493-1.00005-3
Szulc-Musioł, B., Dolińska, B., Kołodziejska, J., & Ryszka, F. (2017). Influence of plasma on the physical properties of ointments with quercetin. Acta Pharmaceutica, 67(4), 569-578. https://doi.org/10.1515/acph-2017-0038 DOI: https://doi.org/10.1515/acph-2017-0038
Voigt, R. (1994). Introduction to pharmaceutical technology (5th ed.). Yogyakarta: Gadjah Mada University Press.
Wazyka, A., Anggrahini, S., & Santoso, U. (2004). Antioxidative properties of white saffron extract (Curcuma mangga Val.) in the β-carotene bleaching and DPPH-radical scavenging methods. Indonesian Food and Nutrition Progress, 11(1), 35-40. https://doi.org/10.22146/jifnp.36
Yang, L., Wen, K. S., Ruan, X., Zhao, Y. X., Wei, F., & Wang, Q. (2018). Response of plant secondary metabolites to environmental factors. Molecules, 23(4), Article 762. https://doi.org/10.3390/molecules23040762 DOI: https://doi.org/10.3390/molecules23040762
Yasir, A. S., Marcellia, S., Wijaya, L. B., & Putri, T. R. (2021). Formulation and activity test of combination gel of ethanol extract of Aloe vera leaves (Aloe vera) and basil leaves (Ocimum sanctum L.) as anti-acne against Staphylococcus epidermidis bacteria. Pharmacoscript, 4(1), 70-86. https://doi.org/10.36423/pharmacoscript.v4i1.610 DOI: https://doi.org/10.36423/pharmacoscript.v4i1.610
Yasir, A. S. (2021). Anti acne gel formulation from combination of basil leaf ethanol extract (Ocimum africanum Lour.) and Aloe vera (Aloe vera (L.) Burm. f.) based on sodium alginate and activity test against Propionibacterium acnes bacteria. Jurnal Farmasi Malahayati, 3(2), 159-173. https://doi.org/10.33024/jfm.v3i2.3801 DOI: https://doi.org/10.33024/jfm.v3i2.3801
Zou, Z., Xi, W., Hu, Y., Nie, C., & Zhou, Z. (2016). Antioxidant activity of Citrus fruits. Food Chemistry, 196, 885-896. https://doi.org/10.1016/j.foodchem.2015.09.072 DOI: https://doi.org/10.1016/j.foodchem.2015.09.072
