Anti-Inflammatory Activity Test fo Ethanol Extract of Kluwih Leaves (Artocarpus camansi Blanco) on Male Wistar White Rats (Rattus norvegicus)

Authors

  • Laela Suci Nofiyanti Universitas Harapan Bangsa
    Indonesia
  • Sunarti Universitas Harapan Bangsa
    Indonesia
  • Galih Samodra Universitas Harapan Bangsa
    Indonesia

DOI:

https://doi.org/10.23917/pharmacon.v21i1.2920

Keywords:

Anti-inflammatory, Artocarpus camansi Blanco, Carrageenan

Abstract

Inflammation occurs when the body reacts to infection, irritation, or other damage. Some compounds in kluwih leaves are believed to have anti-inflammatory potential, one of which is flavonoids. The aim of this study was to find out the activity and effective dosage of the ethanol extract from kluwih leaves as an anti-inflammatory in male white Wistar rats. The experimental animals were divided into five groups: the negative control group (0.5% Na-CMC), the positive control group (Na-diclofenac), and the three treatment groups were given ethanol extract from kluwih leaves at doses of 175, 350, and 700 mg/KgBW orally. After 30 minutes, the soles of the rats' feet were induced with 0.1 ml of 1% carrageenan subplantarly. Edema volume was assessed every 1 hour for 6 hours using a plethysmometer. The data was statistically analyzed using One-Way ANOVA with LSD post hoc test. The research data shows that the ethanol extract of kluwih leaves at doses 175, 350, and 700 mg/KgBW has anti-inflammatory activity. The effective dose of kluwih leaf ethanol extract was 700 mg/KgBW (28.68%) with the second highest percentage of anti-inflammatory power value after the positive control group (31.75%). The LSD (Least Significant Difference) results indicate that the ethanol extract of kluwih leaf at a dose of 700 mg/KgBW does not show a significant difference in its anti-inflammatory effect with the positive control (0.442>0.05).

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References

Apridamayanti, P., Sanera, F., & Robiyanto, R. (2018). Antiinflammatory Activity of Ethanolic Extract from Karas Leaves (Aquilaria malaccensis Lamk.). Pharmaceutical Sciences and Research, 5(3), 152–158. https://doi.org/10.7454/psr.v5i3.4094

Arifah, Y., Sunarti, & Prabandari, R. (2022). Effect of Striped Flower (Clitoria ternatea L.) Against Total Cholesterol, LDL, HDL in Rats (Rattus norvegicus). Journal Syifa Sciences and Clinical Research, 4, 18–31. https://doi.org/10.37311/jsscr.v4i1.13493

Astika, R. Y., Sani, F., & Elisma. (2022). Anti-Inflammatory Activity Test of Cinnamon Leaf Ethanol Extract (Cinnamomum burmanni) In Male White Mice. Jurnal Ilmiah Manuntung, 8(1), 14–23. https://doi.org/10.51352/jim.v8i1.465

Batmomolin, P., Pelu, A. D., & Buton, A. (2022). Anti-Inflammatory Pharmacological Test of Sea Waru Leaf Ethanol Extract (Thespesia populnea (L.) Soland) on White Rats (Rattus norvegicus). Jurnal Pengabdian Ilmu Kesehatan, 2(2). https://doi.org/10.55606/jpikes.v2i2.1276

Cahyaningsih, E., Yuda, P. E. S. K., & Susanthi, I. M. (2018). Test of Antiinflammatory Effect of Ethanol Extract Leaves Salam India (Murraya koenigii L.) Against Rat (Rattus norvegicus) Males Induced By Caragenan 1%). Jurnal Ilmiah Medicamento, 4(1), 25–31. https://doi.org/10.36733/medicamento.v4i1.875

Dewi, N. P., Astari, N. K. A., & Tuldjanah, M. (2022). Test of the Effectiveness of Ethanol Extract of Kluwih Leaves on Histopathological Features of the Liver of White Rats with Hypercholesterolemia Diabetes. Farmakologika Jurnal Farmasi, 19(2). https://doi.org/10.56730/farmakologika.v19i2.489

Dwitiyanti, Astuti, R. D., & Hayati. (2022). Anti-Inflammatory Activity Test of Ethanol Extract 70% Leaves Of Capitals (Sandoricum koetjape (Burm.f.) Merr.) In Male Mice (Mus musculus) With Carrageenan-Induction. Jurnal Ilmiah Kefarmasian, 7(2), 367–380. https://doi.org/10.37874/ms.v7i2.358

Fitriyanti, Hikmah, N., & Astuti, K. I. (2020). Anti-inflammatory Effects of Asoka Flower Infusion (Ixora coccinea l.) on Carrageenan Induced Male Rats. Jurnal Sains Dan Kesehatan, 2(4), 355–359. https://doi.org/10.25026/jsk.v2i4.177

Haqqi, A. N., & Wahyuni. (2023). Effectiveness Test of Ethanol Extract Combination of African Leaves (Vernonia amygdalina Del.) and Kluwih Leaves (Artocarpus camansi) on Reducing Uric Acid Levels in Mice (Mus musculus). Pharmacy Genius, 02(01), 1–12. https://doi.org/10.56359/pharmgen.v2i1.168

Hilaliyah, R. (2021). Utilization of Wild Bandotan Plants (Ageratum conyzoides L.) as a Traditional Medicine and Its Pharmacological Activity. Bioscientiae, 18(1), 28–36. https://doi.org/10.20527/b.v18i1.4065

Islami, D., Pratiwi, D., Zulkifli, & Mardhiyani, D. (2022). Phytochemical Screening of Turmeric (Curcuma domestica Val) and Red Ginger (Zingiber officinale Var Roscoe) Rhizomes Infusion. Jurnal Proteksi Kesehatan, 11(1), 1–6. https://doi.org/10.36929/jpk.v11i1.432

Kementerian Kesehatan RI. (2017). Indonesian Herbal Pharmacopoeia Second Edition. Jakarta, Kementerian Kesehatan RI.

Khatami, M., Farhan, R., & Hafiz. (2022). Anti-inflammatory Effectiveness Test of Pining Bawang (Hornstedtia alliacea) Extract Gel on Mice (Mus musculus) Induced by 1% Carrageenan. Forte Jurnal, 2(2), 120–128. https://doi.org/10.51771/fj.v2i2.328

Lallo, S., Hardianti, B., Umar, H., Trisurani, W., & Wahyuni, A. (2020). Anti-inflammatory and Wound Healing Activities of Mulberry Barks (Morus alba L.) Extract. Jurnal Farmasi Galenika, 6(1), 26–36. https://doi.org/10.22487/j24428744.2020.v6.i1.14661

Marampa, W. R., Ismail, S., & Amir, M. (2022). Testing the Anti-Inflammatory Effect of Mitragyna (Mitragyna speciosa) Ethanol Extract on Mice (Mus musculus) Using the Xylene Induction Method in Mice Ears. Jurnal Ilmiah Indonesia, 7(6), 6831–6840. https://doi.org/10.36418/syntax-literate.v7i6.7279

Maulana, I., Kurniati Roddu, A., & Suriani. (2020). Test the effectiveness of Petai (Parkia speciosa Hassk) bark extract on mice (Mus musculus) as an anti-inflammatory. Lumbung Farmasi: Jurnal Ilmu Kefarmasian, 1(2), 80. https://doi.org/10.31764/lf.v1i2.2559

Muhammad, I. Y., Saehu, M. S., Ertim, Irma, & Nurhikma. (2021). Antiinflammatory Effects of Fraction From Galing Stem Ethanol Extract (Cayratia trifolia L. Domin) In Vitro. Jurnal Farmasi Sains Dan Praktis (JFSP), 7(3), 289–297. https://doi.org/10.31603/pharmacy.v7i3.6107

Nagara, B. P. S. (2019). The Effect of Kluwih Leaf Extract (Artocarpus camansi) on Decreasing the Activity of White Rats (Rattus norvegicus) Male Wistar Strains Induced by High Doses of Paracetamol. Jurnal Agri-Tek: Jurnal Penelitian Ilmu-Ilmu Eksakta, 20(1), 37–40. https://doi.org/10.33319/agtek.v20i1.36

Novika, D. S., Ahsanunnisa, R., & Yani, D. F. (2021). Anti-Inflammatory Activity of Ethanol Extract of Starfruit Leaves (Averrhoa bilimbi L.) Against Inhibition of Protein Denaturation. Stannum : Jurnal Sains Dan Terapan Kimia, 3(1), 16–22. https://doi.org/10.33019/jstk.v3i1.2117

Nurhidayati, L. G. (2020). Formulation and Physical Properties Test of Sodium Diclofenac Nanoemulsion with Combination Tween 80 and Transcutol. Sainteks, 17(1), 33. https://doi.org/10.30595/sainteks.v17i1.6896

Prayogi, K., Kamu, V. S., & Runtuwene, M. R. J. (2022). Phytochemical Screening and Antioxidant Activity Test of Solvent Fraction from Methanol Extract of Pandan Wangi Stem (Pandanus amaryllifolius Roxb). Jurnal LPPM Bidang Sains Dan Teknologi, 7(2), 1–7. https://doi.org/10.35801/jlppmsains.7.2.2022.46794

Purba, N., Andriani Harianja, B., & Akbar, K. (2022). Anti-Inflammatory Activity Test of Lime Leaf (Citrus aurantiifolia) Ethanol Extract in Male Mice (Mus musculus) Induced Carrageenan on 2022. Jurnal Farmasimed (Jfm), 5(1), 14–21. https://doi.org/10.35451/jfm.v5i1.1233

Rahmawati, N., Rahayu, T. P., & Kiromah, N. Z. W. (2023). Cytotoxic Test of Ethyl Acetate Extract of Melinjo Red Skin (Gnetum gnemon L.) On Artemia salina Leach Shrimp Larvae Using The Brine Shrimp Lethality Test (BSLT) Method. Jurnal Ilmiah Kefarmasian, 6(2), 34–40. https://doi.org/10.36760/jp.v6i2.624

Rusli, Z., Yulianita, & Sitanggang, E. E. (2022). Determination of Flavonoid Levels In Ethanol Extract of Longan Leaves (Dimocarpus logan L.) With Variation of Extraction Methods. Ekologia : Jurnal Ilmiah Ilmu Dasar Dan Lingkungan Hidup, 22(1), 31–36. https://doi.org/10.33751/ekologia.v22i1.5042

Sami, F. J., Nur, S., Ramli, N., & Sutrisno, B. (2017). Test the Antioxidant Activity of Kersen Leaves (Muntingia calabura L.) Using the DPPH (1,1-diphenyl-2 picrylhydrazyl) and Frap (Ferric Reducing Antioxidant Power) Methods. As-Syifaa, 09(02), 106–111. https://doi.org/10.56711/jifa.v9i2.258

Samodra, G., & Kusuma, I. Y. (2021). Test of The Anti-Inflammatory Activity Of a Combination of Ethanol Extract of Kencur Rhizome (Kaempferiae Galangal L.) and Tapak Dara Leaves (Catharanthus Roseus) In Mice. Borneo Journal of Pharmascientech, 05(01), 11–21. https://doi.org/10.51817/bjp.v5i1.372

Santi, I., Putra, B., & Wahyuni, S. (2017). Testing the Effect of Ethanol Extract of Green Grass Jelly Leaves (Cyclea barbata Miers) as an Anti-Inflammation in Carragen-Induced White Rats. Jurnal Ilmiah As-Syifaa, 9(1), 58–66. https://doi.org/10.33096/jifa.v9i1.256

Sari, A. K., Aisyah, N., & Prihandiwati, E. (2020). Determination of Total Phenolic Content of Ethanol Extract 96% Terap Leaves (Artocarpus odoratissimus Blanco) By Visible Spectrophotometry Method. Jurnal Ilmiah Ibnu Sina, 5(1), 171–179. https://doi.org/10.36387/jiis.v5i1.417

Sogandi, S., & Amelia, A. (2020). Antibacterial Potency from Ethanol Extract Leaves of Kluwih (Artocarpus camansi Blanco) against Shigella dysenteriae and Bacillus subtilis. Jurnal Ilmu Dasar, 21(2), 105. https://doi.org/10.19184/jid.v21i2.11568

Sumitra, J., & Pasaribu, E. N. R. (2022). Anti-Inflammatory Excitivity Test Of Sintrong Leaf Ethanol Extract (Crassocephalum crepidiodes) On Male White Mice. Jurnal Farmasimed (Jfm), 5(1), 52–56. https://doi.org/10.35451/jfm.v5i1.1279

Sunarti, & Octavini, P. (2023). Antidiabetic Effect of N-Hexane, Ethyl Acetate, and Water Fractions of Clitoria ternatea L. on Streptozotocin-Nikotinamide Induced Rats. Journal of Pharmaceutical and Sciences, 6(2), 400–408. https://doi.org/10.36490/journal-jps.com.v6i2.96

Takaeb, M. J., & Leo, M. I. (2023). Identification of Secondary Metabolites in Qualline Sopi (SOKLIN) Made With and Without Fermentation in Kualin Village, East Nusa Tenggara. 6(2), 111–116. https://doi.org/10.24246/juses.v6i2p111-116

Wardaniati, I., & Yanti, R. (2018). Test of Antioxidant Activity of Trigona Bee Propolis Ethanol Extract (Trigona itama) Using DPPH Method. Journal Of Pharmacy and Science, 2(1), 14–21. https://doi.org/10.36341/jops.v2i1.1257

Wendersteyt, N. V., Wewengkang, D. S., & Abdullah, S. S. (2021). Antimicrobial Activity Test of Exstracts and Fractions of Ascidian herdmania Momus From Bangka Island Waters Likupang Against The Growth of Staphylococcus aureus, Salmonella typhimurium, and Candida albicans. Pharmacon, 10(1), 706–712. https://doi.org/10.35799/pha.10.2021.32758

Widhihastuti, E., Syahida, N., Widyarini, S., & Fakhrudin, N. (2021). Comparison of the Subchronic Anti-Inflammatory Activity of the Ethanol and Ethyl Acetate Extracts of Breadfruit Leaves (Artocarpus altilis) on CFA-Induced Mice. Indonesian Journal of Chemical Science, 10(1). https://doi.org/10.15294/ijcs.v10i1.46870

Wijaya, H., Novitasari, & Jubaidah, S. (2018). Comparison of Extraction Methods On Sea Rambai Leaf Extract Renderment (Sonneratia caseolaris L. Engl). Jurnal Ilmiah Manuntung, 4(1), 79–83. https://doi.org/10.51352/jim.v4i1.148

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Submitted

2023-10-07

Accepted

2024-04-04

Published

2024-06-30

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Articles