Antioxidant and Total Phenolic Testing of Ethanolic Extract of Lime and Kaffir Lime Peel Waste and Peel Off Mask Formulation

Authors

  • Laili Nailul Muna UIN Sunan Kalijaga, Indonesia
    Indonesia
  • Erna Wulandari UIN Sunan Kalijaga, Indonesia
    Indonesia
  • Farhatul Uyun UIN Sunan Kalijaga, Indonesia
    Indonesia
  • Enny Riyan Hasni UIN Sunan Kalijaga, Indonesia
    Indonesia

DOI:

https://doi.org/10.23917/pharmacon.v22i1.8421

Keywords:

Antioxidant, Citrus aurantifolia peel, Citrus hystrix peel, Pell Off mask, Total phenolic

Abstract

The use of synthetic ingredients in cosmetics causes many side effects such as premature aging, skin irritation, acne, blackheads and so on. So it is necessary to develop cosmetics from natural ingredients, such as using citrus waste to make peel-off masks. Development of peel-off mask preparations using active ingredients from kaffir lime peel and lime peel waste which have antioxidant activity and total phenolic content.   The preparation of peel-off masks from citrus peel waste was carried out by extracting phenolic compounds from citrus peels using the maceration method with ethanol solvent and continued by formulating peel-off mask preparations with PVP and PVA bases.   Furthermore, the peel off mask preparation was tested which included organoleptical, pH, adhesion, spreadability and viscosity tests. The method used to analyze the total phenolic content using Folin Ciocalteau method, while the antioxidant activity analysis was tested with DPPH method. The results of this study showed that the measurement of total phenolic content by Folin Ciocalteau method resulted in a value of 862.05 µg/ml per 1000µg of extract, while the measurement of antioxidant activity by DPPH method using a spectrophotometer at a wavelength of 515.5 nm amounted to 63.13 µg/ml with a positive control of vitamin C of 50.17 µg/ml. The peel off mask developed has good physical properties with the value of the preparation obtained pH 6, the value of spreadability 10.35 ± 0.41 grams.cm/s, the value of stickiness 8.00 ± 0.00 seconds, the value of viscosity 2,110.07 ± 83.97 cPa.

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References

Ajay B, Shyam, A., & P.N, D. K. (2023). A Study On Preparation And Evaluation Of Herbal Peel Off Face Mask. International Journal For Multidisciplinary Research, 5(5), 1–5. https://doi.org/10.36948/ijfmr.2023.v05i05.6812

Ali, M. A. M., & Kaid, B. M. (2024). Development And Characterization Of Peel-Off Gel Masks Incorporating Activated Charcoal And Salicylic Acid For Enhanced Skin Radiance And Development And Characterization Of Peel-Off Gel Masks Incorporating Activated Charcoal And Salicylic Acid For Enhanc. September. https://doi.org/10.5281/zenodo.13294605

Amaliah, R. N., Rahmawanty, D., & Ratnapuri, P. H. (2018). Pengaruh Variasi Konsentrasi PVA dan HPMC Terhadap Stabilitas Fisik Masker Gel Peel-Off Ekstrak Metanol Biji Pepaya (Carica papaya L.). Jurnal Pharmascience, 5(1), 78–85. https://doi.org/10.20527/jps.v5i1.5789

Dewi, N. P. (2020). Uji Kualitatif dan Kuantitatif Metabolit Sekunder Ekstrak Etanol Daun Awar-Awar (Ficus septica Burm.f) dengan Metode Spektrofotometer UV-VIS. Acta Holistica Pharmaciana, 2(1), 16–24.

Dhianawaty, D., & Ruslin. (2015). Kandungan Total Polifenol dan Aktivitas Antioksidan dari Ekstrak Metanol Akar Imperata cylindrica (L) Beauv. (Alang-alang). Majalah Kedokteran Bandung, 47(1), 60–64. https://doi.org/10.15395/mkb.v47n1.398

Euis, R. Y. (2017). Pengantar Radikal Bebas dan Antioksidan. Deepublish.

Fauzi, T. M. (2018). Peran Antioksidan Vitamin C Pada Keadaan Stres Oksidatif Dan Hubungan Dengan Kadar Malondialdehid (MDA) Di Dalam Tubuh. Majalah Ilmiah Methoda, 8(2), 61–67.

Haerani, A., Chaerunisa, A. Y., & Subranas, A. (2018). Artikel Tinjauan: Antioksidan untuk kulit. Farmaka, 16, 135–151.

Haryanto, Y. (2023). Pengaruh Suhu Evaporasi Terhadap Kadar Kurkumin dalam Ekstrak Etanol Kunyit (Curcuma domestica, Val). Seminar Nasional Hasil Penelitian Dan Pengabdian Masyarakat, 185, 185–197.

Indrastuti, N., & Aminah, S. (2020). Potensi Limbah Kulit Jeruk Lokal sebagai Pangan Fungsional. Prosiding Seminar Nasional Teknologi Pangan, 13(2), 122–129.

Jang, H. J., Shin, C. Y., & Kim, K. B. (2015). Safety evaluation of polyethylene glycol (PEG) compounds for cosmetic use. Toxicological Research, 31(2), 105–136. https://doi.org/10.5487/TR.2015.31.2.105

Kartika, G. F. (2010). Pengaruh Peningkatan Konsentrasi Carbopol 940 Sebagai Bahan Pengental Terhadap Viskositas Dan Ketahanan Busa Sediaan Shampoo. Yogyakarta : Universitas Sanata Dharma, 1–65.

Kawiji, K., Khasanah, L. U., Utami, R., & Aryani, N. T. (2015). EKSTRAKSI MASERASI OLEORESIN DAUN JERUK PURUT (Citrus hystrix DC) : OPTIMASI RENDEMEN DAN PENGUJIAN KARAKTERISTIK MUTU (Citrus hystrix DC) Oleoresin: Yield Optimization and Quality Characteristics Examination). Jurnal Agritech, 35(02), 178. https://doi.org/10.22146/agritech.13761

Kurniawan, T. W., & Deglas, W. (2019). PEMANFAATAN KULIT BUAH JERUK MANDARIN (Citrus reticulata) DALAM PEMBUATAN PERMEN JELLY DENGAN VARIASI KONSENTRASI BUBUK AGAR. Agrofood, 1(2), 1–5.

Linda, C., Harahap, F., Diningsih, A., & Silvia, E. (2023). Proceedings of the Tapanuli International Health Conference 2022 (TIHC 2022). In Proceedings of the Tapanuli International Health Conference 2022 (TIHC 2022). Atlantis Press International BV. https://doi.org/10.2991/978-94-6463-032-9

Lumentut, N., Edi, H. J., & Rumondor, E. M. (2020). Formulasi dan Uji Stabilitas Fisik Sediaan Krim Ekstrak Etanol Kulit Buah Pisang Goroho (Musa acuminafe L.) Konsentrasi 12.5% Sebagai Tabir Surya. Jurnal MIPA, 9(2), 42. https://doi.org/10.35799/jmuo.9.2.2020.28248

Marfu’ah, S., Fajaroh, F., Romadhona, W. A., & Dwi Taufina, D. (2020). Aktivitas Ekstrak Kulit Jeruk Manis sebagai Antioksidan dan Toksisitasnya Terhadap Artemia Salina. Journal Cis-Trans), 4(2), 7–14. https://doi.org/10.17977/um0260v4i22020p007

Muna, L. N. (2022). Aktivitas antioksidan ekstrak air daun kelor ( Moringa oleifera ) dengan metode DPPH serta analisis kualitatif kandungan metabolit sekunder. Sasambo Journal of Pharmacy, 3(2).

Nurkhasanah, M. A., Si, A., Mochammad, S., Bachri, S., Si, M., Si, D. S., & Yuliani, M. P. (2023). Antioksidan dan Stres Oksidatif.

Patricia, F. (2007). Idebenone, green tea, and Coffeeberry extract: new and innovative antioxidants. Dermatol Ther. https://doi.org/10.1111/j.1529-8019.2007.00146.x

Permadi, N., Julaeha, E., Rosandi, Y., & Nurzaman, M. (2021). Antioxidant Activity of Non-Volatile Lime (Citrus aurantifolia Swingle) Extract. Jurnal Agrinika : Jurnal Agroteknologi Dan Agribisnis, 5(2), 122. https://doi.org/10.30737/agrinika.v5i2.1919

Piluzza G, B. S. (2011). Correlations between phenolic content and antioxidant properties in twenty-four plant species of traditional ethnoveterinary use in the Mediterranean area. Pharm Biol, 49(3), 240–247. https://doi.org/10.3109/13880209.2010.501083. PMID: 21323476

Pramiastuti, O., Zen, D. A., & Prastiyo, B. A. (2018). Penetapan kadar total fenolik dan uji aktivitas antioksidan ekstrak etanol 96% daun kecombrang (Etlingera Elatior) dengan metode 2,2-Difenil-1- Pikrilhidazil (DPPH). Jurnal Farmasi & Sains Indonesia, 1(2), 42–55. http://journal.akfarnusaputera.ac.id/%0APenetapan

Pratiwi, L., & Wahdaningsih, S. (2018). FORMULASI DAN AKTIVITAS ANTIOKSIDAN MASKER WAJAH GEL PEEL OFF EKSTRAK METANOL BUAH PEPAYA (Carica papaya L.). Jurnal Farmasi Medica/Pharmacy Medical Journal (PMJ), 1(2), 50–62. https://doi.org/10.35799/pmj.1.2.2018.21643

Ratanachamnong, P., Chunchaowarit, Y., Namchaiw, P., Niwaspragrit, C., Rattanacheeworn, P., & Jaisin, Y. (2023). HPLC analysis and in vitro antioxidant mediated through cell migration effect of C.hystrix water extract on human keratinocytes and fibroblasts. Heliyon, 9(2), e13068. https://doi.org/10.1016/j.heliyon.2023.e13068

Reza Fahlevi, Ni Deak Made Santi Diwyarthi, Dito Anurogo, Muhammad Anwari, H. A. H. (2023). Gerontologi. In NBER Working Papers. http://www.nber.org/papers/w16019

Riski, R., Nur, S., & Pabontong, E. (2021). Activity Test and Formulation of Antioxidant Cream from Ethanol Extract Combination Of Several Citrus Fruit Peels (Citrus sp. Journal of Pharmaceutical and Medicinal Sciences, 6(1), 23–30.

Russell-Goldman, E., & Murphy, G. F. (2020). The Pathobiology of Skin Aging: New Insights into an Old Dilemma. American Journal of Pathology, 190(7), 1356–1369. https://doi.org/10.1016/j.ajpath.2020.03.007

Ryanata, E. (2015). PENENTUAN JENIS TANIN DAN PENETAPAN KADAR TANIN DARI KULIT BUAH PISANG MASAK (Musa paradisiaca L.) SECARA SPEKTROFOTOMETRI DAN PERMANGANOMETRI. Jurnal Ilmiah Mahasiswa Universitas Surabaya, 4(1), 1–16.

Sayuti, Kesuma., R. Y. (2015). Antioksidan Alami dan Sintetik. Asosiasi Perguruan Tinggi Indonesia (APPTI), Universitas Andalas, Padang.

Setiawan, F., Yunita, O., & Kurniawan, A. (2018). Uji aktivitas antioksidan ekstrak etanol kayu secang dan FRAP. Media Pharmaceutica Indonesiana, 2(2), 82–89.

Sukmawati, A., Laeha, M. N., & Suprapto, S. (2019). Efek Gliserin sebagai Humectan Terhadap Sifat Fisik dan Stabilitas Vitamin C dalam Sabun Padat. Pharmacon: Jurnal Farmasi Indonesia, 14(2), 40–47. https://doi.org/10.23917/pharmacon.v14i2.5937

Sulastri, A., & Chaerunisaa, A. Y. (2018). Formulasi Masker Gel Peel Off untuk Perawatan Kulit Wajah. Farmaka, 14(3), 17–26.

Suryanto, E., & Wehantouw, F. (2009). Aktivitas Penangkap Radikal Bebas dari Ekstrak Fenolik Daun Sukun (Artocarpus altilis F.). Chemistry Progress, 2(1), 1–7.

Susanty, S., & Bachmid, F. (2016). PERBANDINGAN METODE EKSTRAKSI MASERASI DAN REFLUKS TERHADAP KADAR FENOLIK DARI EKSTRAK TONGKOL JAGUNG (Zea mays L.). Jurnal Konversi, 5(2), 87. https://doi.org/10.24853/konversi.5.2.87-92

Velasco MV, Vieira RP, Fernandes AR, Dario MF, Pinto CA, Pedriali CA, Kaneko TM, B. A. (2014). Short-term clinical of peel-off facial mask moisturizers. Int J Cosmet Sci., 355–360. https://doi.org/10.1111/ics.12133. Epub 2014 May 10. PMID: 24750029.

Vieira, R. P., Fernandes, A. R., Kaneko, T. M., Consiglieri, V. O., Pinto, C. A. S. D. O., Pereira, C. S. C., Baby, A. R., & Velasco, M. V. R. (2009). Physical and physicochemical stability evaluation of cosmetic formulations containing soybean extract fermented by Bifidobacterium animalis. Brazilian Journal of Pharmaceutical Sciences, 45(3), 515–525. https://doi.org/10.1590/S1984-82502009000300018

Wahyono, P., Soetjipto, Harjanto, & Suhariningsih. (2011). Efek Jus Buah Tomat (Lycopersicum pyriforme) terhadap Pencegahan Fotoaging Kulit Akibat Iradiasi Sinar Ultraviolet-B. Jurnal Biosains, 13(3), 169–178. http://www.eskom.co.za/CustomerCare/TariffsAndCharges/Documents/RSA Distribution Tariff Code Vers 6.pdf%0Ahttp://www.nersa.org.za/

Wibawa, J. C., Wati, L. H., & Arifin, M. Z. (2020). Mekanisme Vitamin C Menurunkan Stres Oksidatif Setelah Aktivitas Fisik. JOSSAE : Journal of Sport Science and Education, 5(1), 57. https://doi.org/10.26740/jossae.v5n1.p57-63

Widia, I., Abdassah, M., Chaerunisaa, A. Y., Rusdiana, T., Farmasi, F., & Padjadjaran, U. (2018). Farmaka Farmaka. Farmaka, 15(1), 37–46.

Wulan, W., Yudistira, A., & Rotinsulu, H. (2019). UJI AKTIVITAS ANTIOKSIDAN DARI EKSTRAK ETANOL DAUN Mimosa pudica Linn. MENGGUNAKAN METODE DPPH. Pharmacon, 8(1), 106. https://doi.org/10.35799/pha.8.2019.29243

Wulansari, E., Siswanto, A., & Diniatik, D. (2024). Formulasi Tea Tree Oil sebagai Pengawet dalam Masker Gel Peel-Off Ekstrak Etanol Beras Merah (Oryza rufipogon Griff.). JPSCR: Journal of Pharmaceutical Science and Clinical Research, 9(1), 11. https://doi.org/10.20961/jpscr.v9i1.78349

Yumas, M. (2016). FORMULASI SEDIAAN KRIM WAJAH BERBAHAN AKTIF EKSTRA METANOL BIJI KAKAO NON FERMENTASI (Theobroma cacao L) KOMBINASI MADU LEBAH. Jurnal Industri Hasil Perkebunan, 11(2), 75. https://doi.org/10.33104/jihp.v11i2.3414

Yusharyahya, S. N. (2021). Mekanisme Penuaan Kulit sebagai Dasar Pencegahan dan Pengobatan Kulit Menua. EJournal Kedokteran Indonesia, 9(2), 150. https://doi.org/10.23886/ejki.9.49.150

Zaujah, A., Amal, A. S. S., & Marfuah, N. (2020). FORMULASI MASKER GEL PEEL-OFF EKSTRAK BEKATUL PADI BERAS MERAH (Oryza nivara). Pharmaceutical Journal of Islamic Pharmacy, 4(1). https://doi.org/10.21111/pharmasipha.v4i1.3946

Zubaydah, W. O., Sitti, Fandinata, & Septi Selly. (2020). Formulasi Sediaan Masker Gel Peel-Off Dari Ekstrak Buah Tomat (Solanum Lycopersicum L .). Journal Syifa Sciences and Clinical Research, 2(September), 73–82.

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Submitted

2025-01-19

Accepted

2025-07-05

Published

2025-06-30

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